NodC-based evaluation of the occurrence of bacteria in nodules of Pisum sativum isolated on YEM agar

Anna Lenart-Boroń , Tadeusz Zając , Piotr Boroń , Agnieszka Klimek-Kopyra


The bacterial nodulation (nod) genes are essential in the formation process of root nodules. This study was aimed to verify the occurrence of nodule-associated bacteria in two pea varieties (“Tarchalska” and “Klif ”) inoculated with Rhizobium inoculants – Nitragine™ and a noncommercial one produced by the Polish Institute of Soil Science and Plant Cultivation (IUNG). The number of colonies isolated on yeast extract mannitol (YEM) agar from the nodules of “Klif ” inoculated with IUNG inoculants was significantly higher than the number of colonies isolated from other variants. Species identification was based on sequencing of 16S rDNA, which revealed that despite careful sterilization of nodules, sequences of other bacterial species were detected. Among them, one sequence belonged to Rhizobium leguminosarum (isolated from IUNG inoculant). To assess the presence of nodulation-capable Rhizobium, amplification of the nodC gene was performed, which revealed that of 29 samples, 19 were positive. The remaining isolates, including reference strain and bacteria isolated from Nitragine™, lacked this gene. The results show that pea nodules harbor a very diverse community of bacteria. The lack of nodC gene in some strains isolated from plants inoculated with Nitragine™ and with IUNG inoculant proves that even if R. leguminosarum are abundant, they may not be efficient in nodulation.
Author Anna Lenart-Boroń (FoAaE / DoM)
Anna Lenart-Boroń,,
- Department of Microbiology
, Tadeusz Zając (FoAE / IoPP)
Tadeusz Zając,,
- Institute of Plant Production
, Piotr Boroń (FoF / FEPI / DoFFMaPoT)
Piotr Boroń,,
, Agnieszka Klimek-Kopyra (FoAE / IoPP)
Agnieszka Klimek-Kopyra,,
- Institute of Plant Production
Other language title versionsNodC-basierte Bewertung des Auftretens von Bakterien in Wurzelknöllchen von Pisum sativum, isoliert auf YEM-Agar
Journal seriesBodenkultur, ISSN 0006-5471, (N/A 20 pkt)
Issue year2019
Publication size in sheets0.5
Keywords in EnglishRhizobium leguminosarum bv. viceae, Pisum sativum L., inoculant, nod genes, 16S rDNA sequencing
ASJC Classification1102 Agronomy and Crop Science; 1103 Animal Science and Zoology; 1111 Soil Science
Internal identifierWRE/2019/64
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
Score (nominal)20
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.128
Citation count*
Additional fields
FinansowanieThe study was supported by the Ministry of Science and Higher Education in Poland (Project no. N N310 151837) and by the statutory measures of the University of Agriculture (DS KM/3158).
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