Assessment of microbiological aerosol concentration in selected healthcare facilities in southern Poland

Joanna Stec , Anna Lenart-Boroń

Abstract

Objectives: This study was aimed to assess the concentration of microbial aerosol and species composition of airborne staphylococci in 10 healthcare facilities in southern Poland including primary healthcare units and hospital wards; and to assess whether the selected components of microbial aerosol pose a threat of severe infections to either patients or the personnel. Methods: The study was conducted at monthly intervals over a period of one year. Air samples were collected by MAS-100 sampler. The number of mesophilic bacteria, mould fungi, actinomycetes and staphylococci was determined on general and selective media. The species identification of staphylococci was conducted using API tests for strains that were pre-selected based on macroscopic and microscopic observations. Results: A total number of 1,584 samples were collected during the sampling period. The numbers of airborne microorganisms varied between the examined premises and between the seasons of the year. The observed differences were statistically significant with one exception for actinomycetes and their differences between the examined premises. The concentrations of mesophilic bacteria varied from 5 to 297 CFU/m3 of air, for Staphylococcus the values ranged from 1 to 96 CFU/m3, for fungi - from 1 to 100 CFU/m3, and the number of actinomycetes ranged from 7 to 321 CFU/m3. Ten species of coagulase-negative staphylococci (CoNS) were identified among 55 isolates with S. saprophyticus and S. warneri being the most frequently detected (n = 14 and 13, respectively). S. haemolyticus, which is one of the most common causal agents of nosocomial infections was observed in four facilities (n = 5). Conclusions: The microbial concentrations varied both between the seasons of the year and between the examined facilities. The highest bioaerosol concentrations were observed in most crowded premises. The identified species of staphylococci, although not typically associated with human infections, are common causal agents of nosocomial infections and infections in immunocompromised people.
Author Joanna Stec (FoBaH)
Joanna Stec,,
- Faculty of Biotechnology and Horticulture
, Anna Lenart-Boroń (FoAaE / DoM)
Anna Lenart-Boroń,,
- Department of Microbiology
Journal seriesCentral European Journal of Public Health, [European Journal of Public Health], ISSN 1210-7778, e-ISSN 1803-1048, (N/A 40 pkt)
Issue year2019
Vol27
No3
Pages239-244
Publication size in sheets0.5
Keywords in Englishairborne microorganisms, healthcare facilities, bioaerosol, microbial aerosol, Staphylococcus spp.
ASJC Classification2700 General Medicine; 2739 Public Health, Environmental and Occupational Health
DOIDOI:10.21101/cejph.a5681
URL https://cejph.szu.cz/artkey/cjp-201903-0012_assessment-of-microbiological-aerosol-concentration-in-selected-healthcare-facilities-in-southern-poland.php?back=%2Fsearch.php%3Fquery%3Dassessment%2Bof%2Bmicrobiological%2Baerosool%26sfrom%3D0%26spage%3D30
Internal identifierWRE/2019/95
Languageen angielski
Score (nominal)40
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.433; WoS Impact Factor: 2018 = 0.636 (2) - 2018=1.038 (5)
Citation count*
Additional fields
FinansowanieThis study was funded by the statutory measures of the University of Agriculture in Krakow, under the grant No. DS 3102/KM.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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