Quantification of plaque area and characterization of plaque biochemical composition with atherosclerosis progression in ApoE/LDLR−/− mice by FT-IR imaging
Tomasz P. Wrobel , Lukasz Mateuszuk , Renata B. Kostogrys , Stefan Chlopicki , Malgorzata Baranska
AbstractIn this work the quantitative determination of atherosclerotic lesion area (ApoE/LDLR(-/-) mice) by FT-IR imaging is presented and validated by comparison with atherosclerotic lesion area determination by classic Oil Red O staining. Cluster analysis of FT-IR-based measurements in the 2800-3025 cm(-1) range allowed for quantitative analysis of the atherosclerosis plaque area, the results of which were highly correlated with those of Oil Red O histological staining (R(2) = 0.935). Moreover, a specific class obtained from a second cluster analysis of the aortic cross-section samples at different stages of disease progression (3, 4 and 6 months old) seemed to represent the macrophages (CD68) area within the atherosclerotic plaque.
|Journal series||Analyst, ISSN 0003-2654, (A 40 pkt)|
|Publication size in sheets||0.5|
|Score|| = 40.0, 26-07-2017, ArticleFromJournal|
= 40.0, 26-07-2017, ArticleFromJournal
|Publication indicators||= 17|
|Finansowanie||This work was supported by the European Union under the European Regional Development Fund (grant coordinated by JCET-UJ, POIG.01.01.02-00-069/09).|
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