Quantification of plaque area and characterization of plaque biochemical composition with atherosclerosis progression in ApoE/LDLR−/− mice by FT-IR imaging

Tomasz P. Wrobel , Lukasz Mateuszuk , Renata B. Kostogrys , Stefan Chlopicki , Malgorzata Baranska

Abstract

In this work the quantitative determination of atherosclerotic lesion area (ApoE/LDLR(-/-) mice) by FT-IR imaging is presented and validated by comparison with atherosclerotic lesion area determination by classic Oil Red O staining. Cluster analysis of FT-IR-based measurements in the 2800-3025 cm(-1) range allowed for quantitative analysis of the atherosclerosis plaque area, the results of which were highly correlated with those of Oil Red O histological staining (R(2) = 0.935). Moreover, a specific class obtained from a second cluster analysis of the aortic cross-section samples at different stages of disease progression (3, 4 and 6 months old) seemed to represent the macrophages (CD68) area within the atherosclerotic plaque.
Author Tomasz P. Wrobel
Tomasz P. Wrobel,,
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, Lukasz Mateuszuk
Lukasz Mateuszuk,,
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, Renata B. Kostogrys (FoFT / DoHN)
Renata B. Kostogrys,,
- Department of Human Nutrition
, Stefan Chlopicki
Stefan Chlopicki,,
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, Malgorzata Baranska
Malgorzata Baranska,,
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Journal seriesAnalyst, ISSN 0003-2654, (A 40 pkt)
Issue year2013
Vol138
No21
Pages6645-6652
Publication size in sheets0.5
DOIDOI:10.1039/c3an01050c
URL http://pubs.rsc.org/en/Content/ArticleLanding/2013/AN/c3an01050c#!divAbstract
Internal identifierWTŻ/2013/43
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 40.0, 26-07-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 26-07-2017, ArticleFromJournal
Publication indicators WoS Citations = 17
Citation count*
Additional fields
FinansowanieThis work was supported by the European Union under the European Regional Development Fund (grant coordinated by JCET-UJ, POIG.01.01.02-00-069/09).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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