Mutations in the HvDWARF, HvCPD and HvBRI1 Genes-Involved in Brassinosteroid Biosynthesis/Signalling: Altered Photosynthetic Efficiency, Hormonal Homeostasis and Tolerance to High/Low Temperatures in Barley

Iwona Sadura , Ewa Pociecha , Michał Dziurka , Jana Oklešťková , Ondřej Novák , Damian Gruszka , Anna Janeczko

Abstract

Brassinosteroids (BR) are steroid phytohormones that are involved in the growth and stress response in plants, but the precise mechanisms of their action are still being discovered. In our study we have used BR-deficient barley mutants 522DK and BW084 (which carry missense mutations in the HvDWARF and HvCPD genes, respectively). We have also used a BR-signalling mutant that harbors missense substitutions in the HvBRI1 gene. Our aim was (1) to find out if the content of phytohormones in the mutants grown at 20 °C is different than in the wild types and whether/how the content of phytohormones changes after plant acclimation at temperatures of 5 °C and 27 °C?, (2) to characterise the effectiveness of the light reactions of photosynthesis of the barley mutants in comparison to wild types at various temperatures, and (3) to verify the impact of mutations on the tolerance of barley to high and low temperatures. Hormonal characteristics of the BR mutants of barley show the complexity of the interactions between BR and other plant hormones that are additionally modified by temperature and possibly by other factors. The results suggest the participation of BR in auxin catabolism. Further, BR appears to play a role in maintaining the ABA–ABAGlc balance. As for the gibberellin content in plants at a temperature of 20 °C, more in-depth studies will be required to explain the contradictory effects regarding the accumulation of GA3, GA4 and GA5, which appears to be dependent on the type of mutation and connected to the BR level. A fast-kinetic chlorophyll a fluorescence analysis has revealed that the mutants had lower values of energy absorption than the wild types, but the values of the energy transferred via the electron-transport chain was maintained at the wild-type level. We presumed that BR are involved in regulating plant acclimation to extreme (low/high) temperatures, thus the BR-deficient and BR-signalling mutants should be less tolerant to low/high temperatures when compared to the wild types. Unexpectedly, all of the mutants showed a higher tolerance to high temperatures than the wild types. The BW084 and BW312 mutants were less tolerant to frost than the wild type, but 522DK had a similar frost tolerance as the reference wild-type cultivar.
Author Iwona Sadura
Iwona Sadura,,
-
, Ewa Pociecha (FoAaE / DoPP)
Ewa Pociecha,,
- Department of Plant Physiology
, Michał Dziurka
Michał Dziurka,,
-
, Jana Oklešťková
Jana Oklešťková,,
-
, Ondřej Novák
Ondřej Novák,,
-
, Damian Gruszka
Damian Gruszka,,
-
, Anna Janeczko
Anna Janeczko,,
-
Journal seriesJournal of Plant Growth Regulation, ISSN 0721-7595, e-ISSN 1435-8107, (N/A 70 pkt)
Issue year2019
Vol38
No3
Pages1062-1081
Publication size in sheets0.95
Keywords in EnglishBarley, Brassinosteroid content, Frost, Heat, Phytohormones, PSII efficiency
ASJC Classification1102 Agronomy and Crop Science; 1110 Plant Science
DOIDOI:10.1007/s00344-019-09914-z
URL https://link.springer.com/content/pdf/10.1007%2Fs00344-019-09914-z.pdf
Internal identifierWRE/2019/90
Languageen angielski
File
Sadura2019_Article_MutationsInTheHvDWARFHvCPDAndH.pdf 8,6 MB
Score (nominal)70
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.043; WoS Impact Factor: 2018 = 2.179 (2) - 2018=2.893 (5)
Citation count*
Additional fields
FinansowanieThe work was funded by a grant from the National Science Centre (2016–2018) No. 2015/17/B/NZ9/01695 (Poland). Support was also provided by the Czech Science Foundation (Grant No. GA17-06613S) for UHPLC–MS/MS analyses of steroids.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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