Mining for Candidate Genes Controlling Secondary Growth of the Carrot Storage Root

Alicja Macko-Podgórni , Katarzyna Stelmach , Kornelia Kwolek , Gabriela Machaj , Shelby Ellison , Douglas Senalik , Philipp W. Simon , Dariusz Grzebelus

Abstract

Background: Diverse groups of carrot cultivars have been developed to meet consumer demands and industry needs. Varietal groups of the cultivated carrot are defined based on the shape of roots. However, little is known about the genetic basis of root shape determination. Methods: Here, we used 307 carrot plants from 103 open-pollinated cultivars for a genome wide association study to identify genomic regions associated with the storage root morphology. Results: A 180 kb-long region on carrot chromosome 1 explained 10% of the total observed phenotypic variance in the shoulder diameter. Within that region, DcDCAF1 and DcBTAF1 genes were proposed as candidates controlling secondary growth of the carrot storage root. Their expression profiles differed between the cultivated and the wild carrots, likely indicating that their elevated expression was required for the development of edible roots. They also showed higher expression at the secondary root growth stage in cultivars producing thick roots, as compared to those developing thin roots. Conclusions: We provided evidence for a likely involvement of DcDCAF1 and/or DcBTAF1 in the development of the carrot storage root and developed a genotyping assay facilitating the identification of variants in the region on carrot chromosome 1 associated with secondary growth of the carrot root
Author Alicja Macko-Podgórni (FoBaH / Department of Plant Biology and Biotechnology)
Alicja Macko-Podgórni,,
- Department of Plant Biology and Biotechnology
, Katarzyna Stelmach (FoBaH / Department of Plant Biology and Biotechnology)
Katarzyna Stelmach,,
- Department of Plant Biology and Biotechnology
, Kornelia Kwolek (FoBaH / Department of Plant Biology and Biotechnology)
Kornelia Kwolek,,
- Department of Plant Biology and Biotechnology
, Gabriela Machaj (FoBaH / Department of Plant Biology and Biotechnology)
Gabriela Machaj,,
- Department of Plant Biology and Biotechnology
, Shelby Ellison - University of Wisconsin-Madison
Shelby Ellison,,
-
, Douglas Senalik - University of Wisconsin-Madison
Douglas Senalik,,
-
, Philipp W. Simon - University of Wisconsin-Madison
Philipp W. Simon,,
-
, Dariusz Grzebelus (FoBaH / Department of Plant Biology and Biotechnology)
Dariusz Grzebelus,,
- Department of Plant Biology and Biotechnology
Journal seriesInternational Journal of Molecular Sciences, ISSN 1422-0067, (N/A 140 pkt)
Issue year2020
Vol21
No12
Pages1-15
Publication size in sheets0.7
Article number4263
Keywords in EnglishAssociation mapping; Daucus carota; DCAF1; BTAF1; root shape; storage root
ASJC Classification2700 General Medicine; 1312 Molecular Biology; 1503 Catalysis; 1604 Inorganic Chemistry; 1605 Organic Chemistry; 1606 Physical and Theoretical Chemistry; 1607 Spectroscopy; 1706 Computer Science Applications
DOIDOI:10.3390/ijms21124263
URL https://www.mdpi.com/1422-0067/21/12/4263/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Mining for Candidate Genes Controlling Secondary Growth of the Carrot Storage Root of 17-06-2020
1,41 MB
Score (nominal)140
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.224; WoS Impact Factor: 2018 = 4.183 (2) - 2018=4.331 (5)
Citation count*
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FinansowanieThis research was funded by the Ministry of Agriculture and Rural Development of Poland from funds for basic research on crop improvement, years 2014–2020.
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