Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid

Przemysław Podstawski , Wojciech Witarski , Tomasz Szmatoła , Monika Bugno-Poniewierska , Katarzyna Ropka-Molik

Abstract

Sarcoids are the most common skin neoplasm in the Equidae family. Sarcoids are benign, but may cause severe damage in affected animals. Due to the high risk of post-treatment recurrence and the lack of an effective method of treatment, it is reasonable to perform studies on the molecular aspects of this neoplasm. Therefore, the present studies analyzed five genes (cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3 and vimentin) related to cell mobility and invasion traits. Primary healthy fibroblasts and sarcoid cells were obtained from skin biopsies. Cell lines were cultured in two different medium types with different concentrations of foetal bovine serum (10% and 0.5% FBS) to study its influence on the analyzed genes. Gene expression was measured using the real-time PCR method. The results showed significant differences in two genes (coronin and vimentin) depending on culture conditions. In conclusion, the results enabled finding two new genes, related to cell motility and invasion traits, in which gene expression is deregulated. Results of the study may put new knowledge into the complexity of the genetic background of this disease and show the importance of further analysis on this subject
Author Przemysław Podstawski (FoAS / Department of Animal Reproduction, Anatomy and Genomics) - The National Research Institute of Animal Production
Przemysław Podstawski,,
- Department of Animal Reproduction, Anatomy and Genomics
, Wojciech Witarski - The National Research Institute of Animal Production
Wojciech Witarski,,
-
, Tomasz Szmatoła (VET / CfEaIM) - The National Research Institute of Animal Production
Tomasz Szmatoła,,
- Center for Experimental and Innovative Medicine
, Monika Bugno-Poniewierska (FoAS / Department of Animal Reproduction, Anatomy and Genomics)
Monika Bugno-Poniewierska,,
- Department of Animal Reproduction, Anatomy and Genomics
, Katarzyna Ropka-Molik - The National Research Institute of Animal Production
Katarzyna Ropka-Molik,,
-
Journal seriesAnimals, ISSN 2076-2615, e-ISSN 2076-2615, (N/A 100 pkt)
Issue year2020
Vol10
No880
Pages1-9
Publication size in sheets0.5
Keywords in EnglishSkin neoplasia; horse; cell physiology; real-time PCR
ASJC Classification3400 General Veterinary; 1103 Animal Science and Zoology
DOIDOI:10.3390/ani10050880
URL https://www.mdpi.com/2076-2615/10/5/880/pdf
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa (CC-BY); after publication
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Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid of 17-06-2020
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Score (nominal)100
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.148; WoS Impact Factor: 2018 = 1.832 (2)
Citation count*
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FinansowanieThis work was financed by project No. ID 297267 “Directions of use and protection of genetic resources of livestock in conditions of sustainable development” from National Research and Development Center (grant number ID 297267) (material collection and cell lines establishment) and by project No. DI2016 012746 “Diamond Grant” from Ministry of Science and Higher Education, Republic of Poland (the gene expression part of the study
Oświadczenie o afiljacjiT. Szmatoła: załączone, afiljacja do UR; P. Podstawski: oświadczenie o afiljacji
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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