Mouse testicular transcriptome after modulation of non-canonical oestrogen receptor activity

Michał Duliban , Artur Gurgul , Tomasz Szmatoła , P. Pawlicki , A. Milon , Zbigniew Arent , Pawel Grzmil , Małgorzata Kotula-Balak , B. Bilińska

Abstract

The aims of this study were to shed light on the role of G-protein-coupled membrane oestrogen receptor (GPER) and oestrogen-related receptor (ERR) in mouse testis function at the gene expression level, as well as the involvement of GPER and ERR in cellular and molecular processes. Male mice were injected (50µg kg−1,s.c.) with the GPER antagonist G-15, the ERRα inverse agonist XCT790 or the ERRβ/ERRγ agonist DY131. Next-generation sequencing (RNA-Seq) was used to evaluate gene expression. Bioinformatic analysis of read abundance revealed that 50, 86 and 171 transcripts were differentially expressed in the G-15-, XCT790- and DY131-treated groups respectively compared with the control group. Annotated genes and their protein products were categorised regarding their associated biological processes and molecular functions. In the XCT790-treated group, genes involved in immunological processes were upregulated. In the DY131-treated group, genes with increased expression were primarily engaged in protein modification (protein folding and small protein conjugation). In addition, the expression of genes recognised as oncogenes, such as BMI1 proto-oncogene, polycomb ring finger (Bmi1) and nucleophosphin 1 (Npm1), was significantly increased in all experimental groups. This study provides detailed information regarding the genetic changes in the testicular transcriptome of the mouse in response to modulation of non-canonical oestrogen receptor activity.
Author Michał Duliban - Jagiellonian University (UJ)
Michał Duliban,,
-
, Artur Gurgul (VET / CfEaIM)
Artur Gurgul,,
- Center for Experimental and Innovative Medicine
, Tomasz Szmatoła (VET / CfEaIM)
Tomasz Szmatoła,,
- Center for Experimental and Innovative Medicine
, P. Pawlicki - Jagiellonian University (UJ)
P. Pawlicki,,
-
, A. Milon - Jagiellonian University (UJ)
A. Milon,,
-
, Zbigniew Arent (UAK)
Zbigniew Arent,,
- University of Agriculture in Krakow
, Pawel Grzmil - Jagiellonian University (UJ)
Pawel Grzmil,,
-
, Małgorzata Kotula-Balak (VET)
Małgorzata Kotula-Balak,,
- University Centre for Veterinary Medicine UJ-UR
, B. Bilińska - Jagiellonian University (UJ)
B. Bilińska,,
-
Journal seriesReproduction Fertility and Development, [Reproduction, Fertility and Development], ISSN 1031-3613, e-ISSN 1448-5990, (N/A 140 pkt)
Issue year2020
Vol32
No10
Pages903-913
Publication size in sheets0.5
Keywords in EnglishG-protein-coupled oestrogen receptor, next-generation sequencing, oestrogen-related receptor, testis
ASJC Classification2743 Reproductive Medicine; 1103 Animal Science and Zoology; 1305 Biotechnology; 1309 Developmental Biology; 1310 Endocrinology; 1311 Genetics; 1312 Molecular Biology
DOIDOI:10.1071/RD20025
URL https://www.publish.csiro.au/rd/RD20025
Languageen angielski
Score (nominal)140
Score sourcejournalList
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.766; WoS Impact Factor: 2018 = 1.723 (2) - 2018=2.021 (5)
Citation count*
Additional fields
FinansowanieThis work was supported by Grants 2015/18/E/NZ4/00519 and 2016/23/B/ NZ4/01788 from the National Science Centre
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?