Biostimulation by methanol enables the methylotrophic yeasts Hansenula polymorpha and Trichosporon sp. to reveal high formaldehyde biodegradation potential as well as to adapt to this toxic pollutant

Paweł Kaszycki , Tomasz Walski , Nancy Hachicho , Hermann Heipieper

Abstract

The methylotrophic yeasts Hansenula polymorpha and Trichosporon sp. revealed enhanced biodegradation capability of exogenously applied formaldehyde (Fd) upon biostimulation achieved by the presence of methanol, as compared to glucose. Upon growth on either of the above substrates, the strains proved to produce the activity of glutathione-dependent formaldehyde dehydrogenase-the enzyme known to control the biooxidative step of Fd detoxification. However, in the absence of methanol, the yeasts' tolerance to Fd was decreased, and the elevated sensitivity was especially pronounced for Trichosporon sp. Both strains responded to the methanol and/or Fd treatment by increasing their unsaturation index (UI) at xenobiotic levels below minimal inhibitory concentrations. This indicated that the UI changes effected from the de novo synthesis of (poly) unsaturated fatty acids carried out by viable cells. It is concluded that the yeast cell response to Fd intoxication involves stress reaction at the level of membranes. Fluidization of the lipid bilayer as promoted by methanol is suggested as a significant adaptive mechanism increasing the overall fitness enabling to cope with the formaldehyde xenobiotic via biodegradative pathway of C1-compound metabolism.
Author Paweł Kaszycki (FoBaH / IoPBaB)
Paweł Kaszycki,,
- Institute of Plant Biology and Biotechnology
, Tomasz Walski
Tomasz Walski,,
-
, Nancy Hachicho
Nancy Hachicho,,
-
, Hermann Heipieper
Hermann Heipieper,,
-
Journal seriesApplied Microbiology and Biotechnology, ISSN 0175-7598, (A 35 pkt)
Issue year2013
Vol97
No12
Pages5555-5564
Publication size in sheets0.3
Keywords in EnglishMethylotrophic yeast, Hansenula polymorpha, Trichosporon sp., Membrane adaptation, Fatty acid unsaturation, Nonconventional yeast, Formaldehyde biodegradation
ASJC Classification2402 Applied Microbiology and Biotechnology; 2700 General Medicine; 1305 Biotechnology
DOIDOI:10.1007/s00253-013-4796-y
URL http://link.springer.com/article/10.1007%2Fs00253-013-4796-y
Internal identifierWBIO/2013/125
Languageen angielski
Score (nominal)35
Score sourcejournalList
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2014 = 1.458; WoS Impact Factor: 2013 = 3.811 (2) - 2013=4.138 (5)
Citation count*
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FinansowanieThis work was partially supported by the European Commission within its Seventh Framework Program Project BACSIN (Contract no. 211684). This work contributed to the CITE Research Programme of the Helm holtz Centre for Environmental Research.
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