Comparison of haploid and doubled haploid sugar beet clones in their ability to micropropagate and regenerate

Magdalena Klimek-Chodacka , Rafał Barański

Abstract

Background: Haploid plant material is considered as recalcitrant to organogenesis, propagation, and maintenance in vitro. However, sugar beet (Beta vulgaris L.) breeders utilizing doubled haploid (DH) technology in their breeding programs indicate that sugar beet haploids may be cultured in vitro as well as diploids. Thus in this paper the in vitro performance of haploid and the doubled haploid sugar beet of various origin was evaluated. The DHs were derived from haploids by diploidization and twelve such haploid and corresponding DH clone pairs were obtained thus the comparison included haploid and DH clones that had identical allelic composition and differed only in their ploidy level. Results: The genotypes differed in shoot morphology and susceptibility to blackening during culture in vitro, but no significant differences were observed between the haploids and DHs. The micropropagation rate was, on average, higher for the haploids than DHs. Viability of the midrib and petiole explants after a 6-week culture was highly genotype dependent, but not affected by explant ploidy level. However, regeneration efficiency depended on both the genotype and ploidy level. The explants of several haploids regenerated more frequently and developed more adventitious shoots than the corresponding DHs thus overall efficiency was higher for haploids. Conclusions: The results obtained indicate that most of the haploids used in the comparison performed similar to or even better than DHs. This suggests that sugar beet haploid material can be successfully used not only for the production of DHs, but also maintained in vitro and utilized in projects requiring haploid tissues as the source material.
Author Magdalena Klimek-Chodacka (FoH / DoGPBaSS)
Magdalena Klimek-Chodacka,,
- Department of Genetics, Plant Breeding and Seed Science
, Rafał Barański ( / / O-KGHiN)
Rafał Barański,,
-
Journal seriesElectronic Journal of Biotechnology, ISSN 0717-3458, (A 15 pkt)
Issue year2013
Vol16
No2
Pages1-10
Publication size in sheets0.5
Keywords in Englishadventitious buds, axillary shoots, Beta vulgaris, organogenesis, ploidy
DOIDOI:10.2225/vol16-issue2-fulltext-3
URL http://www.scielo.cl/scielo.php?pid=S0717-34582013000200001&script=sci_arttext
Internal identifierWBIO/2013/133
Languageen angielski
LicenseJournal (articles only); author's original; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); after publication
Score (nominal)15
ScoreMinisterial score = 15.0, 30-05-2019, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 30-05-2019, ArticleFromJournal
Publication indicators WoS Citations = 5
Citation count*10 (2020-01-17)
Additional fields
FinansowanieThis work was supported by the Polish Ministry of Agriculture and Rural Development (Grant no. 74/HOR hn-801-22/11).
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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