Comparison of haploid and doubled haploid sugar beet clones in their ability to micropropagate and regenerate

Magdalena Klimek-Chodacka , Rafał Barański

Abstract

Background: Haploid plant material is considered as recalcitrant to organogenesis, propagation, and maintenance in vitro. However, sugar beet (Beta vulgaris L.) breeders utilizing doubled haploid (DH) technology in their breeding programs indicate that sugar beet haploids may be cultured in vitro as well as diploids. Thus in this paper the in vitro performance of haploid and the doubled haploid sugar beet of various origin was evaluated. The DHs were derived from haploids by diploidization and twelve such haploid and corresponding DH clone pairs were obtained thus the comparison included haploid and DH clones that had identical allelic composition and differed only in their ploidy level. Results: The genotypes differed in shoot morphology and susceptibility to blackening during culture in vitro, but no significant differences were observed between the haploids and DHs. The micropropagation rate was, on average, higher for the haploids than DHs. Viability of the midrib and petiole explants after a 6-week culture was highly genotype dependent, but not affected by explant ploidy level. However, regeneration efficiency depended on both the genotype and ploidy level. The explants of several haploids regenerated more frequently and developed more adventitious shoots than the corresponding DHs thus overall efficiency was higher for haploids. Conclusions: The results obtained indicate that most of the haploids used in the comparison performed similar to or even better than DHs. This suggests that sugar beet haploid material can be successfully used not only for the production of DHs, but also maintained in vitro and utilized in projects requiring haploid tissues as the source material.
Autor Magdalena Klimek-Chodacka (WO / O-KGHiN)
Magdalena Klimek-Chodacka
- Katedra Genetyki, Hodowli i Nasiennictwa
, Rafał Barański ( / / O-KGHiN)
Rafał Barański
- Katedra Genetyki, Hodowli i Nasiennictwa
Tytuł czasopisma/seriiElectronic Journal of Biotechnology, ISSN 0717-3458, (A 15 pkt)
Rok wydania2013
Tom16
Nr2
Paginacja1-10
Objętość publikacji w arkuszach wydawniczych0.5
Słowa kluczowe w języku angielskimadventitious buds, axillary shoots, Beta vulgaris, organogenesis, ploidy
DOIDOI:10.2225/vol16-issue2-fulltext-3
URL http://www.scielo.cl/scielo.php?pid=S0717-34582013000200001&script=sci_arttext
Identyfikator wewnętrznyWBIO/2013/133
Języken angielski
LicencjaCzasopismo (tylko dla artykułów); autorska oryginalna; Uznanie Autorstwa - Użycie Niekomercyjne - Bez utworów zależnych (CC-BY-NC-ND); po opublikowaniu
Punktacja (całkowita)15
PunktacjaPunktacja MNiSW = 15.0, 30-05-2019, ArticleFromJournal
Punktacja MNiSW (2013-2016) = 15.0, 30-05-2019, ArticleFromJournal
Wskaźniki publikacji Cytowania WoS = 5
Liczba cytowań*11 (2020-08-08)
Pola dodatkowe
FinansowanieThis work was supported by the Polish Ministry of Agriculture and Rural Development (Grant no. 74/HOR hn-801-22/11).
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* Podana liczba cytowań wynika z analizy informacji dostępnych w Internecie i jest zbliżona do wartości obliczanej przy pomocy systemu Publish or Perish.
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