Udział histaminy w czynności jajnika kury domowej (Gallus domesticus)

Helena Paczoska-Eliasiewicz


In mammals, histamine plays a role in many of the events occuring in the ovary. In birds, studies of ovarian histamine are limited to a brief report concerned with the determination of histamine concentration in three largest preovulatory and postovulatory follicles. In the initial experiments of the ar present study, immuno-reactive histamine was found to be present in all compartments of the ovary of a laying hen., i.e_ in stroma containing follicles with a diameter < I. mm, small white (1-4 tram), large white (4-8 mm),atretic white, yellow preovulatory F7-F1 (8-32 mm) and postovulatory P1-P5 follicles. In the wall of white and yellow follicles, histamine was localised in all layers. i.e. in epithelium with loose connective tissue, theca and granulosa. Therefore, the question has arisen whether histamine is involved in avian ovarian function, Histamine was determined speetrofluorometrically iii stroma and ovarian follicles during: I) sexual maturation, 2) lay, and 3) pause in lay induced by methallibure (I.C.I 33, 828; antigonadot.rophic agent), tamoxifen (I.C.I 46, 474; antiestrogens agent) or forced moult combined with food and water deprivation. In addition, the effect of histamine on: 1) blood flow through stroma and ovarian fbilicles, and 2) secretion in vitro of progesterone, testosterone and estradiol by all populations of ovarian follicles was examined, In vivo, the steroids content of the wall of yellow preovulatory follicles after administration of H1 (terfenadine) and H2 (raniticline) histamine receptor blockers was determined, Blood flow was measured by using the isotopic method, Steroids were assayed radioimmunologically. In a laying hen ovary the concentration of histamine ranged from 6.1 ± 0,33 to 0.36 ± 0.028 1.tg/g tissue and was correlated with the number of mast cells. The highest histamine concentration was exhibited by stroma with follicles 1 mm. while the lowest one - by yellow preovulatory follicle's_ Similar distribution of ovarian histamine was observed during sexual maturation. In the course of follicular development, both at the Lime of sexual maturation and lay, the concentration of histamine in the wall of growing follicles gradually decreased. in the twulatory cycle, changes in the histamine content of the wall Layers of preovulatory follicles shown characteristic rhythmicity which was different for each follicular layer. During the last 24 hours before ovulation the histamine concentration and content of the wall of the largest preovulatory F I follicle significantly increased, reaching the highest levels just before ovulation. After ovulation the histamine level decreased; in the postovul.atory P1 follicle it was significantly lower than in the preovulatory Fl follicle, In the postovulatory P1—P5 follicles, the -histamine concentration gradually increased with progressing regression of these follicles, During the pause in lay connected with the regression of the ovary, the histamine concentration in stroma and ovarian follicles was significantly higher in non-laying than in laying hens. The increase in blood flow through stroma and ovarian follicles following the administration of histamine (5 ug/kg body weight) was not the result of the increased blood cardiac output but the consequence of the local histamine influence on blood flow through the ovary. In vitro, histamine (I ng/m1) significantly affected the secretion of progesterone, testosterone and estradiol by ovarian 161licles, The effect of histamine on the in uitro steroids secretion by the granulosa and theca layers of yellow proovulatory F3—Fl fbilicles changed during the ovulatory cycle, and its stimulatory or inhibitory action was dependent on; 1) the follicular layer secreting steroids, 2) the position of the follicle in the 'hierarchy, and ) the time to follicle ovulation, hi vivo, in the wall of the largest preovulatory Fl Collide the content of progesteron decreased, while that of testosterone and estradiol increased after the. administration or histamine receptor blockers. The results obtained in this study indicate that histamine plays a physiological role in the ovary of the domestic hen. It is suggested that histamine acting as an intraovarian regulator of blood flow and steroidogenesis participates in the processes taking place during growth, maturation and regression or ovarian follicles.
Other language title versionsParticipation of histamine in ovarian function of the domestic hen (Gallus domesticus)
Book typeMonograph
Author Helena Paczoska-Eliasiewicz (UAK)
Helena Paczoska-Eliasiewicz,,
- University of Agriculture in Krakow
PublisherUniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, MNiSW [80]
Publishing place (Publisher address)Kraków
Issue year1999
Book series /Journal (in case of Journal special issue)Zeszyty Naukowe Akademii Rolniczej im. H. Kołłątaja w Krakowie. Rozprawy, ISSN 1233-4189, (0 pkt)
Publication size in sheets6
Languagepl polski
Score (nominal)20
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