Zmienność podatności gatunków i linii pomidorów na zakażenie wirusem mozaiki tytoniu wywołana równoczesnym zakażeniem wirusami TSWV i WXZ

Henryk Jaros

Abstract

The mechanical inoculation with the Tobacco Mosaic Virus of the three species of tomatoes, namely L.hirsutum, L.peruvianum, L.chilense and of the six breeding lines, considered by their growers to be resistant from that disease, led to the following results: the three mentioned above species and four tomato lines specified with the symbols D, H, 0, W did not show any traces of susceptibility to the mechanical infection. The two lines C and E, however, showed a positive response on the tested plant and to the serum anti -TMV only in the inoculated leaves, still after 48 days since their inoculation. Thaus in these cases the TMV found in the inoculated leaves did not move to the other parts of the plant, showing no symptoms on them. The mechanical infection with the Potato Virus X of the investigated collection of tomatoes demonstrated that L.hirsutum and three lines: C, D, W were affected by the systemic invasion and only the line C was previously shown to have a certain degree of susceptibility to the Tobacco Mosaic Virus. Infecting the investigated collection of tomatoes with the inoculum, which was a mixture of the Tobacco Mosaic Virus and Potato Virus.X, gave interesting results. The presence of both the Potato Virus X and Tobacco Mosaic Virus was found throughout the whole plant Lycopersicon hirsutum /A/, which is resistant to TMV. The severe symptoms resulted from the complex action of both viruses. After the simultaneous inoculation with PVX of the two lines C and E, in which a weak response to TMV was observed, the Tobacco Mosaic Virus was noticed throughout the whole plant. The symptoms were severe and were not different from those observed in the greenhouse varieties which were susceptible to both viruses. The experiments in which the TMV and Tomato Spotted Wilt Virus /TSWV/ were simultaneously inoculated, demonstrated the presence of the Tobacco Mosaic Virus throughout the whole plant, only in the lines C and E, which were not completely resistant. It should be emphasized tint the Tomato Spotted Wilt Virus is a spherical virus, different in its structure and characteristics from the bacilliform Tobacco Mosaic Virus and the Potato Virus X. This is probably why the TSWV did not cause the infection with the TMV, in L.hirsutum, which was not the case with the Po¬tato Virus X. The grafting inoculation of scion on stock was carried out in order to find out whether the tomato species and lines showing the highest degree of resistance to TMV, cannot be infected with that virus under different conditions. The scions of the resistant species L.peruvianum, L.chilense and the lines 0 and W were grafted to the TMV infected greenhouse varieties of tomatoes, which served as the stock. As a result of numerous experiments it was concluded that the Tobacco Mosaic Virus transfered to the resistant scion only in the line W. The grafting of the healthy scions taken from the investigated collection of tomatoes on double /TMV + PVX/ infected stocks, demonstrated the observed phenomenon of losing the genetic resistance to TMV infection under the influence of the simultaneous infection of the species L.hirsutum with the Potato Virus X. The phenomenon was also observed, though not so distinctly, in the resistant line W /17-3-1-1-BK ACE MStW/ bred by Walter from the species L.hirsutum. The susceptibility of the lines C and E to the TMV infection, as well as the complete immune resistance of Lycopersicon chilense and the line 0 to that virus were also confirmed. The elektron microscopic investigations were carried out in Uppsala in Sweden. By means of the DIP method it was shown that in the juices from the lines 0, W, C and E, which were mechanically inoculated only with TMV, no forms of that virus were found. With the double infection /TMV + PVX/ of the mentioned lines the Potato Virus X was found in the specimen derived from the line W, whereas in the specimens derived from the lines C and E both the PVC and a small amount of TMV were observed. The transfer of the virus in the tomatoes C and E, which showed a certain degree of resistance, brings about the changes of the infectiousness of the virus under the influence of the resistant host-plant. It is demonstrated, on the one hand, by the increased infectiousness in relation to some plants more resistant than the lines C and E, namely to: L.hirsutum /A/ and the line W. On the other hand the lines and varieties susceptible to the infection with the W. The multiplying 114V in the single cells of the leaves in the tomatoes resistant to TMV, is also observed in the form of the normal and reduced particles of smaller size. Both the experiments carried out by means of inoculation and inoculation by grafting of scion on stock, especially the latter one, revealed in the plants the existence of resistance, shown by the inhibition of translocation of the virus in the plant tissues and not by lack of infection in single cells. In the reported experiments on tomatoes the Potato Virus X /PVX/ seems to act as a factor freeing the transfer of the Tobacco Mosaic Virus through the plasmodesm and the vessel bundles.
Other language title versionsChangeability of susceptibility to the tobacco mosaic virus infection of tomatoes caused by simultaneous infection with TSWV and PVX
Book typeMonograph
Other categories ROZPRAWA_HABILITACYJNA
Author Henryk Jaros (UAK)
Henryk Jaros,,
- University of Agriculture in Krakow
PublisherUniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, MNiSW [80]
Publishing place (Publisher address)Kraków
Issue year1977
Book series /Journal (in case of Journal special issue)Zeszyty Naukowe Uniwersytetu Rolniczego im. Hugona Kołłątaja w Krakowie. Rozprawy, ISSN 1899-3486, (0 pkt)
Vol138
No53
Pages44
Publication size in sheets2.4
Languagepl polski
Score (nominal)20
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