Wybrane substancje biologicznie czynne produkowane przez mikroorganizmy prokariotyczne w różnych środowiskach glebowych górskich ekosystemów trawiastych
AbstractMicrobiological research was carried out in the years 1972-1974 in three experimental plots typical of plant communities similar to the Gladiolo-Agrostietum community and Arrhenatheretum elatioris community belonding to Field Research Centre Institute of Melioration of Grassland in Jaworki and also in the expereimental plot typical for plant community similar to Agrostetum vulgaris unit in Moszczaniec. Fertilization experiments were carried out on the basis of students chasing diffrent plots, using the following fertilizer combinations: 1/ control; 2/ %ON; 3/ P80K80N60; 4/ N60; 5/ P80K80N120; 6/ N120; 7/ F80.480N180; 8/ N180; 9/ P80K80N240; 10/ N240. Fertilizers were used containing super basic phosphate, potash salt and, saltpetre /amunonium nitrate/. In microbiological experiments a method of dilution according to Koch was used, taking into consideretion a general number of microorganisms as well as physiological gropus of bacteria ative in nitrogen and carbohydrate metabolism and also at initiation of mineral phosphorus. Clear cultures were isolated from soil diffrent habitats and were studied in detail. Evaluation was made of the number of bacteria in examined soil samples as well as denoting of chosen biologically active substances such as: free amino acids, amino acids in hydrolized protein and also of riboflavine. The method of thin-layer chromatography and an automatic analyser of Beckman Spinko 120 B were used for aminoacid determination. Determination of riboflavine was carried out in a microbiological way. Agrotechnioal estimation of hay yield was also carried out. On the basis of obtained results it was discovered that under the Influence of mineral fertilization /mainly high nitrogen doses/ there is an increase in the number of microorganisms. Biochemical activity increases /minerelization of organic combinations, ammonitication and nitrification/. Applied mineral fertilization had a noticable effect on the inorease in the number of bacteria of the Azotobacter type on the plant community similar to the Arrhenatheretum elatioris unit. From examinations carried out, the occurence of bacteria of the Arthrobacter type in soil .samples coming from the plant community Agrostetum vulgaris /M/ resulted in high nitrogen dosagen of 180-240 kg/ha with the addition of PK. They caused a decrease of Arthrobacter bacteria. The results of the momentary state of the number of heterotropic bacteria in the examined experimental plots indicate, thatmineral fertilization with high nitrogen doses and the addition of PK gives the highest increase in the number of heterotrophic bacteria. Applied mineral fertilization contributes to an increase in the number free amino acids in examined soil habitats and their composition was dependent on the period of vegetation, the soil and plant cover. In samples of heterotrophic bacteria amino acids were much more abundant than free amino acids. Under the infuence of mineral fertilization, containing a large amount of nitrogen 240 kg/ha with the addition of PK there is a marked increase in the amount of riboflavine A much larger amount of riboflavine was found in samples of bacteria coming from fertilizer combinations of high nitrogen content when compared to control samples. Mineral fertilieiation under favourable meteorological conditions to flueneed an increase in examined grassland productivity. Hay yields evaluated as dry mass increased by 300-400 % wtih N240P80K80 compared to the control of the plant comunity which is similar to Agrosterm vulgaris plant community in Moszczaniec, which was unchanged with mans activity and about a 100 % increase at the experimental plots in Jaworki. The obtained results also proved to be benefical from an agrotechnical point of view because of beneficial changes in the botanical, oompositon of ground cover.
|Other language title versions||The selected active biological substances produced by the procariotic microorganisms in various soil conditions of the mountain grass ecosystems|
|Publisher||Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, MNiSW |
|Publishing place (Publisher address)||Kraków|
|Book series /Journal (in case of Journal special issue)||Zeszyty Naukowe Uniwersytetu Rolniczego im. Hugona Kołłątaja w Krakowie. Rozprawy, ISSN 1899-3486, (0 pkt)|
|Publication size in sheets||4.02|
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