Osadnictwo na Podhalu : geneza i rozwój ukształtowania przestrzennego : (skrót pracy habilitacyjnej)
AbstractAn attempt was made to work out a technique of isolating protein from the four selected varieties of honey corresponding to the Polish Norm requirements: honeydew, nectar multiflorous, buckwheat and rape honeys. In addition, protein extracts from three different anatomical parts of the bees as well as from buckwheat and rape pollen were made. It was found that depending on the honey variety, after dialysis, only 12,2 % - 37,0 % of protein /initial amount/ were stated in the extracts. All the isolated proteins obtained were separated by polyscrylamide gel disc electrophoresis. General variations were fond in electrophoretio patterns, although corresponding fractions were stated for honey and bee proteins as well as for buckwheat honey protein and buck¬wheat pollen. No corresponding fraotione however, were ascertained for rape pollen and honey. With a view to study the immunogenic properties of honey proteins and those originating from the bees, rabbits were immunized by two techniques. With the use of antisera in aerological reactions it was stated that the proteins of the studied honeys and those originating from the bees revealed antigenic properties, In the cross reactions of double gel diffusion, corresponding protein fractions were found for the studied bees and variety honeys as well as for the buckwheat pollen and honey. No rape pollen proteins in the rape honey were detected. The allergenic aotivity of honey antigens was tested on the rabbits immunized with honey protein. Statistical analysis of the results revealed that with respect to en early reaction, only antigens "5" /from honeydew / and "GR" / from buckwheat honey/ were found to display a statistically significant action. Antigen "N" / from nectar multiflorous honey / showed an early reaction but it was not statistically significant. As concerns a joint reaction of antigen 7N" viz., the early and later ones treated together, it was found to be statistically significant. Within the later reaction the allergenic activity of antigens “N" and "RZ" /from rape honey/ was not found to be statistically significant, however it was approaching the critical value. It can be inferred that with the more numerous material used the studied reactions would prove significant. The antigens of the honeydew and multiflorous honeys were investigated in the Clinic of Allergy and Immunology, Medical Academy in Krak6w, in 30 patients with bronchial astha and in 3 healthy people. On the basis of statistical analysis it has been found that both the honeydew and nectar multiflorous honeys are powerful allergens particularly for allergic patients.
|Other language title versions||Studies on the immunologically active substances in honey|
|Publisher||Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, MNiSW |
|Publishing place (Publisher address)||Kraków|
|Book series /Journal (in case of Journal special issue)||Zeszyty Naukowe Uniwersytetu Rolniczego im. Hugona Kołłątaja w Krakowie. Rozprawy, ISSN 1899-3486, (0 pkt)|
|Publication size in sheets||3.2|
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