Przydatność kultur sterylnych do rozmnażania wiśni oraz wartość produkcyjna otrzymanych tą metodą drzew
AbstractStudies concerning the micropropagation of sour cherry were carried out on the cultivars English Morello, North Star and Kelleris 16. The shoots were propagated in the Murashige and Skoog medium with a modified concentration of auxins and cytokinin. They were subcultured 10 times at intervals of 3-4 weeks and each time the number of shoots suitable for Waller proliferation (non-vitrified shoots) was recorded. Atter dividing the shoots into two length groups: 0,3-1 cm — rosettes, and above 1 cm — long shoots, the number of vitrified shoots was counted. Irrespective of cultivar, the greatest rate of multiplication (5.2-6,7 shoots after one month of culture) was obtained in the MS medium supplemented with 0.1 mg/I IBA and 1.0 mg/1 BAP. No vitrified shoots were obtained in the MS medium with the addition of 0.1 mg/I NAA and 0.25 mg/I BAP. Using MS with 0.1 mg/1 IAA and 0.25 mg/1 BAP, and MS with 0.1 mg/1 IBA and 0.25 mg/I BAP gave 0.24 and 0.36 vitrified shoots, respectively. The number of shoots 0.3-1 cm in length (suitable for futher proliferation) and above 1 cm (suitable fiar rooting) differed according to the hormone composition of medium and the cultivar. The evaluation of TC (tissue-cultured) trees for orchard production was carried out in two orchards. The first experiment (Brzezna, 1987-1994) included three sour cherry cultivars: English Morello, North Star and Nefris. The English Morello cultivar was represented by three types: one from Skierniewice (L/Sk/v) and two from Krakow (1./Kr 5/v and L/Kr 2/v). Prunus inahaleb was the rootstock for English Morello trees and the F12/1 clone of Prunus avian'. was the rootstock for North Star trees. The trees of Nefris cultivar originated only from sterile cultures. The percentage of fruit-set, yield, mass of 100 fruits, dimensions of trees (area of the cross-section of a trunk, A and B, and crown volume) enabling the calculation of the productivity indexes (in kg/cm2 and kg/m3), and sums of root diameters were evaluated in the field experiment carried out in 1987-1994. In the case of the English Morello cultivar the trunk cross-section areas, sum of root diameters, percentage of fruit-set, and mass of 100 fruits and stones were similar for the TC trees and the trees grafted on the rootstock, while the yield and the productivity index were greater in the TC trees. The results obtained for the North Star cultivar followed a different pattern: the trunk cross-section area (A) and its increments (B), fruit-set, yield, and mass of 100 fruits and stones were greater in the trees growing on the rootstock than in those propagated in vitro. The TC trees of North Star cultivar, however, had the same (in kg/m3) or slightly higher (in kg/cm2) productivity index than the trees growing on the rootstock. The second field experiment (Garlica Murowana, 1992-1997) was carried out on two sour cherry cultivars. The trees originated from sterile cultures or were bud-grafted on the F12/1 rootstock. The same parameters of tree growth and fruiting were recorded as in the first experiment. For the English Morello cultivar no differences in the investigated characteristics were found between the trees cultured in vitro and those growing on the rootstock. The TC trees of North Star cultivar yielded better and had a higher productivity index than the trees grafted on the rootstock. Good productivity indexes of the tissue-cultured trees, obtained in both orchards, indicate that trees propagated by this method have a great commercial value.
|Other language title versions||The usefulness of sterile cultures in sour cherry micropropagation and the productivo v211,09 of trees obtained by this method|
|Publisher||Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, MNiSW |
|Publishing place (Publisher address)||Kraków|
|Book series /Journal (in case of Journal special issue)||Zeszyty Naukowe Akademii Rolniczej im. H. Kołłątaja w Krakowie. Rozprawy, ISSN 1233-4189, (0 pkt)|
|Publication size in sheets||3.5|
|Citation count*||2 (2016-09-06)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.