Funkcjonalne oddziaływanie na organizm szczurów nasion fasoli (Phaseolus vulgaris L.) jako składnika diety wysokofruktozowej
AbstractRecent decades have seen a change in the model of human nutrition, consisting in a significant increase in the consumption of carbohydrates, among them fructose. The cause of this phenomenon lies in the rising levels of fast-foods, especially carbonated soft-drinks, in the everyday diet. The average consumption of fructose has increased as much as 80-fold, from ca. 0.5 to 40 g/day, over the last 30 years. During this period, a large body of evidence has been collected on the detrimental effect of high intakes of fructose, especially in the absence of antioxidants. Food products that contain significant amounts of antioxidants, including beans, may prevent harmful changes in the organism, caused by oxidative stress. Most researches into the impacts of fructose on organisms have been based on a high-fructose model with the fructose content of ca. 60%. Reaching such a large percentage of fructose in the human diet, however, seems impossible even if one's dietary habits are bad; therefore, the present study was aimed to investigate the effect of a lower dose of fructose in the everyday diet. The other objective was to determine whether cooked beans can prevent the undesirable changes that may be induced by the elevated consumption of fructose. Nutritional experiments were performed on male Wistar rats during the period of 5 or 9 weeks. The study material consisted of the following: (i) cooked beans (Phaseolus vulgaris L.), var. Toffi, previously selected from five new varieties as one with highest antioxidant activity; (ii) diets fed to the laboratory rats, containing 31% fructose, and/or 15.5% and/or 31% cooked bean seeds; and (iii) blood and internal organs of animals after the end of the experiments. The bean seeds and the diets were characterised taking into account chemical composition, phenolic content and quality, and antioxidant activity. The following parameters were measured in the tissues of rats: the concentration of a-tocoferol and malondialdehyde (MDA) in blood plasma; the basic biochemical parameters of blood plasma; the susceptibility of red blood cells to the oxidation with cumene hydroperoxide; the activity of superoxide dismutase (SOD) in red blood cells; the activity of catalase (CAT) in organ homogenates; and the activity of glutation peroxidase (GPx) in plasma and internal organs. The results of the study allowed formulation of the following conclusions: 1. The consumption of diets containing 31% (wow) of fructose caused a slight but statistically significant increase (as compared to the control group) in plasma glucose and a decrease in the activity of alkaline phosphatase in laboratory rats, while not influencing the other biochemical parameters of blood. 2. Fructose intake did not have a clear impact on blood lipids, except for plasma triglycerides whose level significantly increased both in the 9-week experiment (a < 0.05) and the 5-week test (a < 0.1). 3. Regardless of the duration of experiments, consuming diets with 31% of fructose promoted the oxidative breakdown of lipids. 4. Fructose consumed in the diet did not influence the reducing capacity of blood plasma (as measured by FRAP method) or the activity of plasma peroxidase in rats. Similarly, this dietary component had a nonsignificant impact on the activity of glutatione peroxidase and catalase in the internal organs of rats. 5. Consuming diets with 31% of fructose for either 5 or 9 weeks did not cause any visible symptoms of the metabolic syndrome or oxidative stress in the rat organism. 6. Regardless of the amount of cooked beans in the diet, the consumption of bean--containing diets strongly and significantly influenced the blood lipids of rats, reducing the concentration of lipoproteins (total cholesterol, HDL and LDL) and triglycerides. The effect was stronger for the higher dose of beans in the diet. 7. Cooked beans consumed in the diet decreased the oxidative damage to blood lipids. The blood plasma of rats receiving diets with beans showed a higher ratio of vitamin E to trigicerides and had significantly lower levels of malondialde¬hyde. 8. The consumption of cooked beans in the diet increased the activity of glutathione peroxidase (GPx) and catalase (CAT) in most of the internal organs of rats. The plasma activity of peroxidase (GPx3) was not significantly affected by the consumed beans in the 5-week experiment, but was significantly lowered in the 9-week test. The antioxidant capacity of blood plasma was not influenced by beans. 9. The two diet components investigated in the study produced clearly antagonistic effects on the blood plasma lipids of rats. The negative influence of fructose, causing the concentration of plasma triglicerides to increase, was inhibited by cooked beans.
|Other language title versions||Functional influence on rat organism of been (Phaseolus vulgaris L.) seeds as a component of high-fructose diet|
|Publisher||Uniwersytet Rolniczy im. Hugona Kołłątaja w Krakowie, MNiSW |
|Publishing place (Publisher address)||Kraków|
|Book series /Journal (in case of Journal special issue)||Zeszyty Naukowe Uniwersytetu Rolniczego im. Hugona Kołłątaja w Krakowie. Rozprawy, ISSN 1899-3486, (0 pkt)|
|Publication size in sheets||7.5|
|Keywords in Polish||stres oksydacyjny, fruktoza, Phaseolus vulgaris, badania żywnościowe, szczury|
|Keywords in English||oxidative stress, fructose, Phaseolus vulgaris, feeding experiments, rats|
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